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Histological evaluation at different times after augmentation of extraction sites grafted with a magnesium‐enriched hydroxyapatite: double‐blinded randomized controlled trial
Author(s) -
Canullo Luigi,
Heinemann Friedhelm,
Gedrange Tomasz,
Biffar Reiner,
KunertKeil Christiane
Publication year - 2013
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1111/clr.12035
Subject(s) - alkaline phosphatase , masson's trichrome stain , tartrate resistant acid phosphatase , chemistry , trichrome , h&e stain , extraction (chemistry) , immunohistochemistry , medicine , andrology , pathology , acid phosphatase , chromatography , biochemistry , enzyme
Objectives To histologically analyze the early angiogenesis–osteogenesis interplay in post‐extraction sockets augmented with magnesium‐enriched hydroxyapatite ( M g‐enriched HA ). Material and Methods Ten post‐extraction sites underwent post‐extraction ridge preservation procedure. According to randomization, sites were divided into two balanced groups and bone specimens were collected 2 or 4 months after surgery. Sections were stained with hematoxylin/eosin, M asson– G oldner trichrome, and tartrate‐resistant acid phosphatase ( TRAP ), respectively. Furthermore, indirect immunohistochemistry was performed using alkaline phosphatase, CD 34 and caveolin‐1 antibodies. Mean values and standard deviations were calculated for each outcome variable. Data were then compared using one‐way ANOVA test. P < 0.05 was considered statistically significant. Results Histomorphometric analysis presented 15.0% (±3.5) regenerated bone after 2 months of healing. After 4 months, regenerated bone increased 5.1‐fold up to 77.4% (± 8.6) ( P < 0.001). On the contrary, vessels and capillary reduced from 645 (±33) to 255 (± 94) (caveolin‐1 expression, P = 0.008). These findings were confirmed by CD 34 expression (301 ± 95 and 88 (±24), respectively, at 2 and 4 months ( P = 0.046). Conclusions Within the limits of the present randomized controlled study, it can be concluded that M g‐enriched HA is a suitable material for socket preservation and ensures early angiogenesis and early osteogenesis.