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Effects of titanium oxide surface properties on bone‐forming and soft tissue‐forming cells
Author(s) -
Wheelis Sutton E.,
MontañoFigueroa Ana Gabriela,
QuevedoLopez Manuel,
Rodrigues Danieli C.
Publication year - 2018
Publication title -
clinical implant dentistry and related research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.338
H-Index - 85
eISSN - 1708-8208
pISSN - 1523-0899
DOI - 10.1111/cid.12656
Subject(s) - viability assay , oxide , titanium , crystallinity , anodizing , materials science , alkaline phosphatase , titanium oxide , osseointegration , morphology (biology) , in vitro , chemistry , biophysics , biomedical engineering , chemical engineering , implant , metallurgy , biochemistry , composite material , surgery , medicine , enzyme , aluminium , biology , organic chemistry , genetics , engineering
Background Previous studies have concluded that certain titanium oxide (TiO 2 ) surface properties promote bone‐forming cell attachment. However, no comprehensive studies have investigated the effects of TiO 2 surface and film morphology on hard and soft tissues. Purpose The aim of this study is to understand the effects of TiO 2 morphology on the proliferation and differentiation of murine preosteoblasts (MC3T3‐E1) and proliferation of human gingival fibroblasts (HGF‐1) using in vitro experiments. Materials and Methods Samples were fabricated with several TiO 2 thickness and crystalline structure to mimic various dental implant surfaces. in vitro analysis was performed for 1, 3, and 7 days on these samples to assess the viability of MC3T3‐E1 and HGF‐1 cells in contact with the modified oxide surfaces. Results Results showed that HGF‐1 cells exhibited no significant difference in viability on modified oxide surfaces versus a titanium control across experiments. MC3T3‐E1 cells exhibited a significantly higher viability for the modified oxide surface in 1 day experiments, but not in 3 or 7 day experiments. Alkaline phosphatase expression in MC3T3‐E1 was not significantly different on modified oxide surfaces versus the control across all experiments. A slight positive trend in viability was observed for cells in contact with rougher modified oxide surfaces versus a titanium control in both cell types. Conclusions These observations suggest that crystallinity and thickness do not affect the long‐term viability of hard or soft tissue cells when compared to a cpTi surface. Therefore, treatments like anodization on implant components may not directly affect the attachment of hard or soft tissue cells in vivo.