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Adhesion Properties of Human Oral Epithelial‐Derived Cells to Zirconia
Author(s) -
Okabe Eijiro,
Ishihara Yuichi,
Kikuchi Takeshi,
Izawa Ario,
Kobayashi Shuichiro,
Goto Hisashi,
Kamiya Yosuke,
Sasaki Keisuke,
Ban Seiji,
Noguchi Toshihide,
Kawai Tatsushi,
Mitani Akio
Publication year - 2016
Publication title -
clinical implant dentistry and related research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.338
H-Index - 85
eISSN - 1708-8208
pISSN - 1523-0899
DOI - 10.1111/cid.12369
Subject(s) - cubic zirconia , titanium , cell adhesion , hemidesmosome , integrin , laminin , adhesion , epithelium , materials science , cell , microbiology and biotechnology , chemistry , medicine , biology , pathology , biochemistry , composite material , ceramic , metallurgy
Background Few studies have examined epithelial attachment to zirconia and the proliferative ability of epithelial cells on zirconia surfaces. Purpose To evaluate the adhesion properties of zirconia materials for epithelial cell attachment and compare this with titanium and alumina. Materials and Methods Human oral epithelial cells were cultured on smooth‐surfaced specimens of commercially pure titanium ( cpTi ), ceria‐stabilized zirconia/alumina nano‐composite ( P ‐ NANOZR ), yttria‐stabilized zirconia ( C ercon), and alumina oxide (in C oris AL ). The cell morphology, the cell viability and mRNA of integrin β 4 , laminin γ 2 , catenin δ 2 , and E ‐cadherin were evaluated by SEM , C ell‐ C ounting K it‐8, and real‐time PCR , respectively. Results Morphology of cells attached to specimens was similar among all groups. The viable cell numbers on C ercon and in C oris AL after 24 hours culture were significantly higher than for cpTi . Integrin β 4 , laminin γ 2 , and catenin δ 2 mRNA expression was not different among all groups. However, at 3 and 24 hours after incubation, E ‐cadherin mRNA expression in the P ‐ NANOZR group was significantly higher than for cpTi . Conclusion Zirconia may support binding of epithelial cells through hemidesmosomes comparable with titanium. Furthermore, P ‐ NANOZR may impart resistance to exogenous stimuli through strong intercellular contacts with peri‐implant mucosal cells when used as an abutment and implant superstructure.