Premium
Peri‐Implant Bone Regeneration Using rhPDGF‐BB , BMSCs , and β‐TCP in a Canine Model
Author(s) -
Xu Ling,
Zhang Wenjie,
Lv Kaige,
Yu Weiqiang,
Jiang Xinquan,
Zhang Fuqiang
Publication year - 2016
Publication title -
clinical implant dentistry and related research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.338
H-Index - 85
eISSN - 1708-8208
pISSN - 1523-0899
DOI - 10.1111/cid.12259
Subject(s) - osseointegration , regeneration (biology) , alkaline phosphatase , dentistry , implant , biomedical engineering , medicine , chemistry , microbiology and biotechnology , surgery , biology , biochemistry , enzyme
Background The presence of insufficient bone volume remains a major clinical problem for dental implant placement to restore oral function. Tissue engineering provides a promising approach for inducing bone regeneration and enhancing osseointegration in dental implants. Purpose The tissue‐engineered bone consisting of recombinant human platelet‐derived growth factor ( rhPDGF‐BB ), bone marrow stem cells ( BMSCs ), and beta‐tricalcium phosphate ( β‐TCP ) particles was validated for the first time in a preclinical large animal canine model in terms of its ability to promote new bone formation around the implants, as well as osseointegration between the tissue‐engineered bone and dental implants. Materials and Methods Proliferation and osteogenic differentiation of canine BMSCs treated with rhPDGF‐BB were evaluated with an MTT , alkaline phosphatase ( ALP ) activity, A lizarin R ed staining, and real‐time quantitative PCR ( RT‐qPCR ) analysis of osteogenic genes. The therapeutic potential of tissue‐engineered bone consisting of rhPDGF‐BB / BMSCs / β‐TCP in bone repair was evaluated in mesial‐implant defects of immediate postextraction implants in the canine mandible. Results rhPDGF‐BB treatment significantly increased proliferation and osteogenic differentiation of canine BMSCs . Furthermore, the tissue‐engineered bone consisting of rhPDGF‐BB / BMSCs / β‐TCP significantly enhanced bone formation and osseointegration. Conclusion This study provides important evidence that supports the potential application of rhPDGF‐BB / BMSCs / β‐TCP tissue‐engineered bone in immediate implantation for oral function restoration.