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Influence of the Periodontal Status on the Initial‐Biofilm Formation on Titanium Surfaces
Author(s) -
MartínezHernández Miryam,
OlivaresNavarrete René,
AlmaguerFlores Argelia
Publication year - 2016
Publication title -
clinical implant dentistry and related research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.338
H-Index - 85
eISSN - 1708-8208
pISSN - 1523-0899
DOI - 10.1111/cid.12251
Subject(s) - biofilm , titanium , dentistry , materials science , chemistry , medicine , biology , bacteria , metallurgy , genetics
Background Dental implants will be exposed to a complex ecosystem once they are placed in the oral cavity. The bacterial colonization and biofilm formation on these devices will depend not only on the physicochemical surface implant properties but also on the periodontal health conditions of the patients, as these devices are exposed. Purpose The aim of this study was to correlate the subgingival microbial profile with the composition of initial biofilm formed on different microstructured titanium ( T i) surfaces. Materials and Methods Ten periodontitis and 10 periodontally healthy subjects were included in this study. The subjects wore a removable acrylic device with four different fixed T i surfaces for 48 hours. Microbial samples of subgingival plaque and the biofilm formed on each T i surface were individually analyzed by the checkerboard DNA‐DNA hybridization technique. Results Despite the roughness or hydrophilicity of the T i surfaces, a characteristic pattern of bacterial adhesion was observed on each of the study groups. However, significant differences in the proportion of the species that colonized the T i surfaces were found between the periodontitis and periodontally healthy groups. T reponema denticola , N eisseria mucosa , E ikenella corrodens , and T annerella forsythia were detected in higher proportions on the T i disks placed in the periodontitis subjects, while significant higher proportions of C apnocytophaga sputigena , F usobacterium periodonticum , P revotella melaninogenica , and S treptococcus mitis were detected on the T i disks placed in the periodontally healthy group. Conclusions The results obtained in this study shows that the composition and the proportion of the species that initially colonize T i surfaces are highly influenced by the periodontal status more than the surface characteristics of the T i implant.