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Agreement between Histomorphometry and Microcomputed Tomography to Assess Bone Microarchitecture of Dental Implant Sites
Author(s) -
Dias Danilo Rocha,
Leles Cláudio Rodrigues,
Batista Aline Carvalho,
Lindh Christina,
RibeiroRotta Rejane Faria
Publication year - 2015
Publication title -
clinical implant dentistry and related research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.338
H-Index - 85
eISSN - 1708-8208
pISSN - 1523-0899
DOI - 10.1111/cid.12176
Subject(s) - bone histomorphometry , nuclear medicine , x ray microtomography , implant , trabecular bone , dental implant , medicine , dentistry , materials science , biomedical engineering , pathology , osteoporosis , surgery , radiology
Abstract Background: Histomorphometry and microcomputed tomography ( microCT ) have been used in implant studies but need better understanding before being used as equivalent methods. Purpose The purpose of this study was to investigate the agreement between 2D (histomorphometry) and 3D ( microCT ) reference methods for assessing jawbone microarchitecture in vivo .Material and Methods Forty‐four bone specimens from 32 patients were obtained during implant placement and examined by microCT , followed by hematoxylin–eosin staining and histomorphometric analysis. The morphometric parameters included bone volume density ( BV / TV ), bone surface fraction ( BS / TV ), bone surface density, trabecular thickness, trabecular number, and trabecular separation ( T b. S p). Bland– A ltman plots were used for pairwise agreement analysis between the equivalent 3D and 2D parameters, and complemented with M ountain plots. The association between the two methods was tested using Pearson's correlation followed by P assing– B ablok regression. Results Systematic bias was observed in all B land– A ltman and M ountain plots, including constant bias for BV / TV and T b. S p, and proportional bias for all other parameters. Significant correlation was found for BV / TV ( r = 0.80; p < .001) and BS / TV ( r = 0.44; p = .003), and the P assing– B ablok regression showed constant bias for BV / TV and proportional bias for BS / TV . Conclusion Because of the poor agreement between measures obtained by histomorphometry and microCT , these methods should not be used interchangeably for jawbones.