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A Novel BMP2 ‐Coprecipitated, Layer‐by‐Layer Assembled Biomimetic Calcium Phosphate Particle: A Biodegradable and Highly Efficient Osteoinducer
Author(s) -
Zheng Yuanna,
Wu Gang,
Liu Tie,
Liu Yi,
Wismeijer Daniel,
Liu Yuelian
Publication year - 2014
Publication title -
clinical implant dentistry and related research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.338
H-Index - 85
eISSN - 1708-8208
pISSN - 1523-0899
DOI - 10.1111/cid.12050
Subject(s) - layer (electronics) , phosphate , calcium , bone morphogenetic protein 2 , particle (ecology) , materials science , chemical engineering , nanotechnology , biophysics , chemistry , biochemistry , metallurgy , in vitro , engineering , oceanography , geology , biology
Purpose To repair large‐size bone defects, most bone‐defect‐filling materials in clinic need to obtain osteoinductivity either by mixing them with particulate autologous bone or adsorbing bone morphogenetic protein 2 ( BMP2 ). However, both approaches encounter various limitations. In this study, we hypothesized that our novel particles of biomimetic BMP2 ‐coprecipitated calcium phosphate ( BMP2‐cop.BioCaP ) could serve as an independent and biodegradable osteoinducer to induce bone formation efficiently for these bone‐defect‐filling materials, for example, deproteinized bovine bone ( DBB ). Materials and Methods We alternately layer‐by‐layer assembled amorphous and crystalline CaP triply to enable a “bamboo‐like” growth of the particles. We functionalized BioCaP by coprecipitating BMP2 into the most outer layer of BioCaP . We monitored the degradation, osteoinductivity, and foreign‐body reaction of either BMP2‐cop.BioCaP or its combination with DBB in an ectopic site in rats. Results After 5 weeks, the BMP2‐cop.BioCaP significantly induced new bone formation not only alone but also when mixed with DBB . Its osteoinductive efficiency was 10‐fold higher than the adsorbed BMP2 . Furthermore, BMP2‐cop.BioCaP also reduced significantly the host foreign‐body reaction to DBB in comparison with the adsorbed BMP2 . After a 5‐week implantation, more than 90% of BMP2‐cop.BioCaP degraded. Conclusions These findings indicate a promising clinical potential for BMP2‐cop.BioCaP in the repair of large‐size bone defects.