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Using zebrafish in systems toxicology for developmental toxicity testing
Author(s) -
Nishimura Yuhei,
Inoue Atsuto,
Sasagawa Shota,
Koiwa Junko,
Kawaguchi Koki,
Kawase Reiko,
Maruyama Toru,
Kim Soonih,
Tanaka Toshio
Publication year - 2016
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/cga.12142
Subject(s) - zebrafish , developmental toxicity , toxicity , model organism , biology , organism , toxicology , animal testing , teratology , computational biology , bioinformatics , medicine , genetics , pregnancy , gestation , gene
With the high cost and the long‐term assessment of developmental toxicity testing in mammals, the vertebrate zebrafish has become a useful alternative model organism for high‐throughput developmental toxicity testing. Zebrafish is also very favorable for the 3R perspective in toxicology; however, the methodologies used by research groups vary greatly, posing considerable challenges to integrative analysis. In this review, we discuss zebrafish developmental toxicity testing, focusing on the methods of chemical exposure, the assessment of morphological abnormalities, housing conditions and their effects on the production of healthy embryos, and future directions. Zebrafish as a systems toxicology model has the potential to elucidate developmental toxicity pathways, and to provide a sound basis for human health risk assessments.