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Simple in vitro migration assay for neural crest cells and the opposite effects of all ‐trans ‐retinoic acid on cephalic‐ and trunk‐derived cells
Author(s) -
Usami Makoto,
Mitsunaga Katsuyoshi,
Irie Tomohiko,
Miyajima Atsuko,
Doi Osamu
Publication year - 2014
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/cga.12059
Subject(s) - neural crest , retinoic acid , in vitro , microbiology and biotechnology , trunk , biology , chemistry , cell culture , biochemistry , genetics , embryo , botany
Here, we describe a simple in vitro neural crest cell ( NCC ) migration assay and the effects of all ‐trans ‐retinoic acid ( RA ) on NCCs . Neural tubes excised from the rhombencephalic or trunk region of day 10.5 rat embryos were cultured for 48 h to allow emigration and migration of NCCs . Migration of NCCs was measured as the change in the radius (radius ratio) calculated from the circular spread of NCCs between 24 and 48 h of culture. RA was added to the culture medium after 24 h at embryotoxic concentrations determined by rat whole embryo culture. RA (10 μM) reduced the migration of cephalic NCCs , whereas it enhanced the migration of trunk NCCs , indicating that RA has opposite effects on these two types of NCCs .