Simple  in vitro  migration assay for neural crest cells and the opposite effects of all ‐trans ‐retinoic acid on cephalic‐ and trunk‐derived cells | Zendy

Usami Makoto | Zendy; Mitsunaga Katsuyoshi | Zendy; Irie Tomohiko | Zendy; Miyajima Atsuko | Zendy; Doi Osamu | Zendy

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Simple in vitro migration assay for neural crest cells and the opposite effects of all ‐trans ‐retinoic acid on cephalic‐ and trunk‐derived cells

Author(s) -

Usami Makoto,

Mitsunaga Katsuyoshi,

Irie Tomohiko,

Miyajima Atsuko,

Doi Osamu

Publication year - 2014

Publication title -

congenital anomalies

Language(s) - English

Resource type - Journals

eISSN - 1741-4520

pISSN - 0914-3505

DOI - 10.1111/cga.12059

Subject(s) - neural crest , retinoic acid , in vitro , microbiology and biotechnology , trunk , biology , chemistry , cell culture , biochemistry , genetics , embryo , botany

Here, we describe a simple in vitro neural crest cell ( NCC ) migration assay and the effects of all ‐trans ‐retinoic acid ( RA ) on NCCs . Neural tubes excised from the rhombencephalic or trunk region of day 10.5 rat embryos were cultured for 48 h to allow emigration and migration of NCCs . Migration of NCCs was measured as the change in the radius (radius ratio) calculated from the circular spread of NCCs between 24 and 48 h of culture. RA was added to the culture medium after 24 h at embryotoxic concentrations determined by rat whole embryo culture. RA (10 μM) reduced the migration of cephalic NCCs , whereas it enhanced the migration of trunk NCCs , indicating that RA has opposite effects on these two types of NCCs .

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