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Wnt11 expression in rat dental pulp and promotional effects of W nt signaling on odontoblast differentiation
Author(s) -
Koizumi Yu,
Kawashima Nobuyuki,
Yamamoto Mioko,
Takimoto Koyo,
Zhou Mengyu,
Suzuki Noriyuki,
Saito Masahiro,
Harada Hidemitsu,
Suda Hideaki
Publication year - 2013
Publication title -
congenital anomalies
Language(s) - English
Resource type - Journals
eISSN - 1741-4520
pISSN - 0914-3505
DOI - 10.1111/cga.12011
Subject(s) - odontoblast , chemistry , pulp (tooth) , microbiology and biotechnology , andrology , dentistry , biology , medicine
Congenital anomalies of wingless‐type mouse mammary tumor virus ( MMTV) integration site family ( W nt) are frequently accompanied with tooth and dentin abnormality. The aim of this study was to investigate the effects of W nt signaling on odontoblast differentiation of mouse dental papilla cells ( MDPs ). Mouse dental papilla cells were cultured in α‐modified minimum essential medium containing 10% fetal bovine serum and antibiotics. Odontoblast differentiation was induced by bone morphogenic protein 2 ( BMP2 ), and the expression of odontoblast‐specific markers and Wnt‐related signaling molecules was analyzed by real‐time reverse transcription‐polymerase chain reaction and immunohistochemistry. Odontoblast differentiation was evaluated by dentin sialophosphoprotein ( D spp ) and dentin matrix protein ( DMP ) 1 expression. Localization of β‐catenin in MDPs was detected by immunocytochemistry using an anti‐β‐catenin antibody. D spp expression in MDPs was upregulated in the presence of BMP2 . W nt5a , W nt11 , L ef1 and T cf4 expression was upregulated in BMP2 ‐treated MDPs . W nt11 expression was detected in rat dental pulp in vivo , and particularly strong expression of W nt11 was detected in odontoblasts. Enhanced D spp and DMP1 expression and alkaline phosphatase activity induced by BMP2 were completely negated by the W nt antagonist: IWR ‐1‐endo treatment. Nuclear translocation of β‐catenin observed in BMP2 ‐treated MDPs was also negated by IWR ‐1‐endo treatment. These results indicate that W nt signaling upregulates odontoblast marker expression in MDPs , suggesting a promoting effect of Wnt signaling on odontoblast differentiation.