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Prognostic significance of polo‐like kinases in retinoblastoma: correlation with patient outcome, clinical and histopathological parameters
Author(s) -
Singh Lata,
Pushker Neelam,
Sen Seema,
Singh Mithalesh K,
Chauhan Feeroj A,
Kashyap Seema
Publication year - 2015
Publication title -
clinical and experimental ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.3
H-Index - 74
eISSN - 1442-9071
pISSN - 1442-6404
DOI - 10.1111/ceo.12517
Subject(s) - retinoblastoma , immunohistochemistry , medicine , polo like kinase , pathology , carcinogenesis , retinoblastoma protein , clinical significance , cell cycle , oncology , biology , cancer , biochemistry , gene
Background Retinoblastoma is evolving, but it is still a therapeutic challenge for pediatric oncologists. Polo‐like kinases ( PLK s) plays an important role in cell cycle events. They play a crucial role in cell proliferation which may lead to tumour formation. The objective of this study is to investigate the role of PLK 1 and PLK 3 proteins in human retinoblastoma tissues. Design Non‐randomized, prospective study was performed in the D r R . P . C entre for O phthalmic S ciences, All India Institute of Medical Sciences, N ew D elhi, I ndia. Participants This study included 74 primary enucleated retinoblastoma tissues. Methods Expression of PLK 1 and PLK 3 protein were assessed in primary enucleated retinoblastoma tissues by immunohistochemistry and western blotting. Main Outcome Measures Expression of PLK 1 and PLK 3 protein were correlated with clinical and histopathological parameters, tumour staging and overall survival of patients. Results Immunohistochemical results revealed expression of PLK 1 in 47/74 (63.51%) cases and PLK 3 in 31/74 (41.89%) cases. Western blotting confirmed the immunoreactivity results. Expression of PLK 1 showed correlation with poor differentiation and tumour invasion. In addition, PLK 1 was statistically significant with massive choroidal invasion, whereas PLK 3 did not correlate with any of the clinical or histopathological parameters. There was no statistical correlation in the overall survival of patients with PLK 1 and PLK 3 expression. Conclusions PLK 1 expression was associated with poor tumour differentiation and histopathological high‐risk factors. These proteins may be involved in tumorigenesis and progression of disease. These results suggest that PLK 1 may act as a potential therapeutic target and a promising marker for developing potent small molecule inhibitors of PLK isoforms in retinoblastoma.