Premium
Safety evaluation of rabbit eyes on scleral collagen cross‐linking by riboflavin and ultraviolet A
Author(s) -
Wang Mengmeng,
Zhang Fengju,
Liu Kegao,
Zhao Xu
Publication year - 2015
Publication title -
clinical and experimental ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.3
H-Index - 74
eISSN - 1442-9071
pISSN - 1442-6404
DOI - 10.1111/ceo.12392
Subject(s) - medicine , erg , ophthalmology , electroretinography , rabbit (cipher) , histology , corneal collagen cross linking , ultraviolet a , ultrastructure , sclera , retina , retinal , anatomy , pathology , cornea , biology , keratoconus , statistics , mathematics , dermatology , neuroscience
Abstract Background To evaluate the safety of the scleral collagen cross‐linking ( CXL ) transferred from corneal CXL in rabbit eyes using histology and electroretinography ( ERG ). Methods Stress–strain measurement was performed five rabbits 1 week after scleral CXL . Dark‐adapted ERG was repeatedly applied on bilateral eyes of 10 rabbits before, and 1 week, 1 month and 3 months after the CXL operation. Histological analysis, terminal deoxynucleotidyl transferase dUTP nick‐end labeling, the measurement of outer nuclear layer thickness and transmission electron microscopy were performed onto 15 rabbits at corresponding time points postoperatively. Results The mechanical and biochemical stability of rabbit scleral tissues was statistically increased after the scleral CXL ( P = .00). However, the dark‐adapted ERG amplitudes were statistically reduced 1 week, 1 month and 3 months postoperatively ( P < 0.05). Compared with the control eyes, apoptotic cells and ultrastructural changes can be found in the retinal layers of scleral cross‐linked eyes. Conclusions According to the electrophysiological and histopathological results, the current scleral CXL laboratory technique is not safe enough for the postoperative visual function of rabbit eyes.