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Reliability of cone counts using an adaptive optics retinal camera
Author(s) -
Bidaut Garnier Mélanie,
Flores Mathieu,
Debellemanière Guillaume,
Puyraveau Marc,
Tumahai Perle,
Meillat Mathieu,
Schwartz Claire,
Montard Michel,
Delbosc Bernard,
Saleh Maher
Publication year - 2014
Publication title -
clinical and experimental ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.3
H-Index - 74
eISSN - 1442-9071
pISSN - 1442-6404
DOI - 10.1111/ceo.12356
Subject(s) - intraclass correlation , repeatability , reproducibility , medicine , coefficient of variation , cone (formal languages) , correlation coefficient , ophthalmology , reliability (semiconductor) , pearson product moment correlation coefficient , nuclear medicine , mathematics , statistics , physics , power (physics) , algorithm , quantum mechanics
Background To assess the reproducibility and repeatability of cone imaging in healthy human eyes, using the RTx ‐1 A daptive O ptics R etinal C amera and its proprietary cone‐counting software. Design Single‐centre, prospective study. Participants Ten healthy adults. Methods Macular cones were imaged. Intrasession repeatability was assessed by comparing 10 consecutive acquisitions obtained by the same operator from each subject. For the intersession study, each subject was imaged five consecutive days. Interoperator reproducibility was also evaluated by comparing the images obtained from 10 different subjects by two independent operators. Finally, intergrader agreement was evaluated by comparing the cone counts measured by two masked graders. Main Outcome Measures Mean cone density (cells/mm 2 ), spacing between cells (μm) and percentage of cones with six neighbours calculated on V oronoi diagrams were measured. Correlation coefficients, intraclass correlation coefficients, and coefficients of variation were calculated. Results Correlation coefficient and intraclass correlation coefficient were respectively 0.81 and 0.96 between operators, and 0.97 and 0.98 between the two graders. The intrasession and intersession coefficients of variation were under 7%. The percentage of cells with six neighbours and the spacing between cones varied in the same proportion (coefficients of variation ranged from 1.66 to 10.05%). Conclusions Overall, the test–retest variability of RTx ‐1 and its software was good in normal human eyes. Further studies in the normal clinical setting are mandatory.