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Long noncoding RNA‐mRNA expression profiles and validation in pancreatic neuroendocrine neoplasms
Author(s) -
Ji Meng,
Tang Liang,
Ding Rumei,
Shi Ligang,
Liu Anan,
Chen Danlei,
Shao Chenghao
Publication year - 2020
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/cen.14156
Subject(s) - biology , long non coding rna , carcinogenesis , microarray , cancer research , messenger rna , microarray analysis techniques , pi3k/akt/mtor pathway , gene expression , signal transduction , microbiology and biotechnology , rna , gene , genetics
Background Pancreatic neuroendocrine neoplasms (pNENs) are a group of endocrine tumours arising in the pancreas and deemed to be the most common neuroendocrine tumours. The pathogenesis of pNENs remains unknown. Long noncoding RNAs (lncRNAs) are aberrantly expressed in cancers. The functional roles of lncRNAs and lncRNA‐mRNA expression profiles in pNENs are undefined. The aim of this study was to identify the lncRNA and mRNA expression profiles and explore the lncRNA‐mRNA co‐expression networks associated with the pNENs carcinogenesis. Method Differentially expressed lncRNA and mRNA in pNENs tissues from adjacent tissues were detected using human lncRNA microarray V3.0 containing 30 586 lncRNA and 26 109 coding transcripts. Probable functions for lncRNAs and mRNAs were predicted according to lncRNA‐mRNA network. Results The microarray identified 2080 lncRNAs and 1771 mRNAs in pNENs tumours differentially expressed compared with the adjacent tissues. The GO terms and KEGG pathway annotation data indicated that cell projection morphogenesis, cell adhesion molecules pathway, PI3K‐AKT signalling pathway, focal adhesion pathway, neuroactive ligand‐receptor interaction pathways and Ras signalling pathways were significantly associated with the pNENs tumorigenesis. Co‐expression network analysis revealed the differential interactions between lncRNAs and mRNAs in pNENs tumours and adjacent tissues. The genes, situated at the important nodes of the co‐expression network, include ICOSLG, ENST512077, FGF8 and ENST511918. Conclusions There were significant differences in lncRNA and mRNA expression between pNEN tumours and adjacent tissues, and these differences were associated with tumorigenesis through multiple biological processes and signalling pathways. These results provided important insights regarding lncRNA in pNENs pathogenesis and provide potential therapeutic targets.