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Leuprolide acetate‐stimulated androgen response during female puberty
Author(s) -
Hernandez María Isabel,
MartinezAguayo Alejandro,
Cavada Gabriel,
Avila Alejandra,
Iñiguez German,
Mericq Veronica
Publication year - 2015
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/cen.12690
Subject(s) - endocrinology , medicine , androstenedione , androgen , testosterone (patch) , dehydroepiandrosterone , basal (medicine) , sex hormone binding globulin , follicle stimulating hormone , adrenarche , insulin , luteinizing hormone , hormone
Summary Background A physiological increase in androgen levels occurs during adolescence. Measuring androgen concentrations is the best method to distinguish normal evolution processes from hyperandrogenic disorders. Hypothesis The increase in circulating androgens during puberty is inversely associated with insulin sensitivity in normal weight girls. Objective To assess circulating levels of ovarian androgens and anti‐Müllerian hormone ( AMH ) at baseline and after Gn RH analogue (Gn RH ‐a) stimulation in normal pubertal girls across different Tanner stages. We also studied the association between this response and insulin sensitivity. Design Prospective study of healthy girls (6–12 years) from the local community ( n = 63). Methods Tanner I ( n = 23) subjects were assessed cross‐sectionally, and Tanner II girls ( n = 40) were evaluated every 6 months until they reached Tanner V. Early morning dehydroepiandrosterone sulphate ( DHEA ‐S), AMH , sex hormone‐binding globulin ( SHBG ), androstenedione, glucose and insulin levels were measured. A Gn RH ‐a test (500 μg/m 2 ; sc) and oral glucose intolerance test ( OGTT ) were performed. Differences throughout puberty were evaluated. Results Basal and/or stimulated Testosterone DHEA‐S and 17‐hydroxyprogesterone (17 OHP ) were inversely associated with insulin sensitivity ( WIBSI ) from the beginning of puberty, whereas androstenedione was directly associated with gonadotrophins. AMH was inversely associated with basal and stimulated gonadotrophins and directly with insulin area under the curve ( AUC ) only in the early stages of puberty. 17 OHP and testosterone responsiveness increased significantly during puberty in all subjects, whereas testosterone levels changed less consistently. This pattern of ovarian‐steroidogenic response was most evident during mid‐ and late puberty. Moreover, during late puberty only, basal 17 OHP , testosterone and DHEA ‐S were positively associated with gonadotrophins. Conclusion In normal nonobese girls born appropriate for gestational age, androgen synthesis was associated with insulin sensitivity in early puberty and with LH only in late puberty.