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Deconvolution‐based assessment of pituitary GH secretion stimulated with GHRH +arginine in P rader‐ W illi adults and obese controls
Author(s) -
Grugni Graziano,
Marostica Eleonora,
Crinò Antonino,
Marzullo Paolo,
Nicolao Giuseppe,
Sartorio Alessandro
Publication year - 2013
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/cen.12142
Subject(s) - medicine , endocrinology , area under the curve , arginine , secretion , biology , amino acid , biochemistry
Summary Objective The assessment of GH deficiency in adult patients with Prader‐Willi syndrome ( PWS ) has been previously assessed through the evaluation of quantitative parameters, such as the peak value of GH response to exogenous stimuli. A comprehensive description of the pattern of secretory response obtainable by deconvolution analysis is still lacking. The aim of our study was to characterize the time evolution of responses of PWS subjects compared with obese controls. Design and subjects GH responsiveness was measured following the combined administration of GHRH +arginine to 65 PWS adults (24 males, 41 females) aged 18–41·2 years, and 17 age‐, gender‐ and body mass index‐matched obese controls. PWS subjects were analysed considering the stratification on different genotypes. Measurements GH response to GHRH +arginine was analysed in terms of peak values, standard area under the curves ( AUC s), AUC s due to the stimulus, AUC s of the Instantaneous Secretion Rate signal and Secretion Response Analysis. Results In terms of both peak values and AUC , GH responses were statistically different between PWS UPD 15 and PWS DEL 15 subjects as well as between PWS UPD 15 and obese controls. PWS subjects showed a lower and a more delayed GH response compared with obese controls. Moreover, PWS UPD 15 subjects had the most delayed GH response. Conclusions Our findings demonstrate that impaired GH secretion in PWS subjects compared with obese controls regards not only amplitude parameters such as peak value and AUC , but also the shape of the secretory response, which is more delayed, especially for UPD 15 subjects.