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The effects of caloric restriction on F etuin‐ A and cardiovascular risk factors in rats and humans: a randomized controlled trial
Author(s) -
Choi Kyung Mook,
Han Kyung Ah,
Ahn Hee Jung,
Lee So Young,
Hwang Soon Young,
Kim BaekHui,
Hong Ho Cheol,
Choi Hae Yoon,
Yang Sae Jeong,
Yoo Hye Jin,
Baik Sei Hyun,
Choi Dong Seop,
Min Kyung Wan
Publication year - 2013
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/cen.12076
Subject(s) - caloric theory , medicine , endocrinology , randomized controlled trial
Summary Objectives The liver‐secreted protein fetuin‐ A is associated with insulin resistance, metabolic syndrome, type 2 diabetes and atherosclerosis. We examined the effect of caloric restriction ( CR ) on fetuin‐ A levels and concomitant changes in hepatic steatosis and cardiovascular risk factors in rats and humans. Design and Subjects We performed a randomized, controlled clinical trial to examine circulating fetuin‐ A levels and cardiovascular risk parameters including visceral fat area ( VFA ), atherogenic lipid profile, inflammatory markers, adipokines levels and brachial artery endothelial function in 76 overweight women with type 2 diabetes before and after 12 weeks of CR . In addition, the effects of CR on hepatic steatosis and fetuin‐ A m RNA expression were evaluated in O tuska L ong E vans T okushima F atty ( OLETF ) rats, an animal model of obesity and type 2 diabetes. Results Circulating fetuin‐ A levels were significantly decreased after 12 weeks of CR and were accompanied by improvements in VFA , blood pressure, glucose, lipid profiles and liver function. The CR group also showed a significant decrease in apolipoprotein B , leptin and insulin resistance compared to those in the control group, although endothelial function was not different. Multiple regression analysis showed that the changes in fetuin‐ A levels were independently associated with CR and changes in hs CRP and adiponectin ( R 2 = 0·156). Moreover, CR significantly reduced hepatic steatosis and fetuin‐ A expression, as well as weight, glucose, total cholesterol and triglyceride levels, in OLETF rats. Conclusion Caloric restriction significantly reduced the hepatic expression of fetuin‐ A and its circulating levels and improved several cardiovascular risk factors in obese rats and humans with type 2 diabetes.