The effect of interleukin (IL)‐21 and CD4 + CD25 ++  T cells on cytokine production of CD4 +  responder T cells in patients with myasthenia gravis | Zendy

AlahgholiHajibehzad M. | Zendy; Durmuş H. | Zendy; Aysal F. | Zendy; GülşenParman Y. | Zendy; Oflazer P. | Zendy; Deymeer F. | Zendy; SaruhanDireskeneli G. | Zendy

Open Access

The effect of interleukin (IL)‐21 and CD4 + CD25 ++ T cells on cytokine production of CD4 + responder T cells in patients with myasthenia gravis

Author(s) -

AlahgholiHajibehzad M.,

Durmuş H.,

Aysal F.,

GülşenParman Y.,

Oflazer P.,

Deymeer F.,

SaruhanDireskeneli G.

Publication year - 2017

Publication title -

clinical & experimental immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.329

H-Index - 135

eISSN - 1365-2249

pISSN - 0009-9104

DOI - 10.1111/cei.13006

Subject(s) - cytokine , myasthenia gravis , immunology , il 2 receptor , interleukin , interleukin 2 , endocrinology , biology , medicine , t cell , immune system

Summary Impairment of the suppressive function of regulatory T (T reg ) cells has been reported in myasthenia gravis (MG). In this study, cytokine‐related mechanisms that may lead to the defect of T reg were investigated in patients with anti‐acetylcholine receptor antibody‐positive MG (AChR + MG). Proliferation and cytokine production of responder T (T resp ) cells in response to polyclonal activation were measured in a suppression assay. The effect of interleukin (IL)‐21 on suppression was evaluated in vitro in co‐culture. IL‐21 increased the proliferation of T resp cells in T resp /T reg co‐cultures. T resp cells from patients with MG secreted significantly lower levels of IL‐2. In patients with MG, IL‐2 levels did not change with the addition of T reg to cultures, whereas it decreased significantly in controls. In T resp /T reg co‐cultures, IL‐4, IL‐6 and IL‐10 production increased in the presence of T reg in patients. Interferon (IFN)‐γ was decreased, whereas IL‐17A was increased in both patient and control groups. IL‐21 inhibited the secretion of IL‐4 in MG and healthy controls (HC), and IL‐17A in HC only. The results demonstrated that IL‐21 enhances the proliferation of T resp cells in the presence of T reg . An effect of IL‐21 mainly on T resp cells through IL‐2 is implicated.

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