VB ‐201, an oxidized phospholipid small molecule, inhibits CD 14‐ and Toll‐like receptor‐2‐dependent innate cell activation and constrains atherosclerosis

Summary Atherosclerosis is an inflammatory disease of the vascular wall. Activated monocytes and dendritic cells ( DC ) in the intima layer of the vasculature promote atherogenesis. Toll‐like receptor ( TLR)‐ 2 and TLR‐ 4, which are predominantly expressed on these cells and mediate their activation, are essential for atherosclerosis development. In this study we demonstrate that VB ‐201, an oxidized phospholipid ( O x‐ PL ) small molecule, inhibits TLR signalling restricted to TLR‐ 2 and TLR‐ 4 in human and mouse monocytes and DC . Mechanistically, we show that VB ‐201 binds directly to TLR‐ 2 and CD 14, the TLR‐ 4 co‐receptor, to impair downstream cues and cytokine production. In a rabbit model, oral administration of VB ‐201 constrained atherosclerosis progression. This effect was not due to reduced cholesterol abundance, as hyperlipidaemia was sustained. We suggest that VB ‐201 may counter inflammation where TLR‐ 2 and/or CD 14 complicity is essential, and is therefore beneficial for the treatment of atherosclerosis.