Premium
PINK1 in normal human melanocytes: first identification and its effects on H 2 O 2 ‐induced oxidative damage
Author(s) -
Wang L.,
Ding X.,
Huang H.,
Li Z.,
Li M.,
Du J.,
Zhang J.
Publication year - 2021
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.14431
Subject(s) - pink1 , oxidative stress , downregulation and upregulation , mitochondrion , microbiology and biotechnology , mitochondrial permeability transition pore , reactive oxygen species , biology , oxidative phosphorylation , apoptosis , intracellular , chemistry , programmed cell death , biochemistry , mitophagy , autophagy , gene
Summary Background Oxidative stress plays an important role in initiating the destruction of melanocytes, which could be one possible mechanism of vitiligo. PINK1 is an outer membrane protein of mitochondria, which protects many cells from oxidative stress through regulating mitochondrial function. However, the role of PINK1 and its effects on oxidative damage in melanocytes have not been elucidated. Aim To investigate the expression and effects of PINK1 on oxidative stress in human melanocytes. Methods Quantitative reverse transcription−PCR and western blot analysis were used to analyse the expression of PINK1 in PIG1 melanocyte and gene downregulation models. Levels of cell viability, cell apoptosis and intracellular reactive oxygen species (ROS), mitochondrial morphology, mitochondrial membrane potential (MMP) and mitochondrial permeability transition pore (MPTP) opening were measured in PIG1 models transfected with PINK1 small interfering RNA with or without hydrogen peroxide (H 2 O 2 ). Results We first observed the expression of PINK1 in human PIG1 melanocytes and found that downregulation of PINK1 made melanocytes more sensitive to oxidative stress induced by H 2 O 2 , with more cell apoptosis and increased intracellular ROS. Meanwhile, downregulation of PINK1 caused morphological changes in mitochondria, decreased the MMP and increased MPTP opening. Conclusions Our study found PINK1 plays an essential role in protecting human melanocytes from oxidative stress.