Premium
Interleukin ( IL )‐8 and IL ‐36γ but not IL ‐36Ra are related to acrosyringia in pustule formation associated with palmoplantar pustulosis
Author(s) -
Xiaoling Y.,
Chao W.,
Wenming W.,
Feng L.,
Hongzhong J.
Publication year - 2019
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.13689
Subject(s) - palmoplantar pustulosis , pathogenesis , psoriasis , medicine , immunohistochemistry , dermis , interleukin , messenger rna , pathology , immunology , dermatology , biology , cytokine , gene , biochemistry
Summary Background Palmoplantar pustulosis ( PPP ) is a refractory, nonbacterial impetigo confined to the palms and soles. Its pathogenesis is still obscure, but it may be associated with the large eccrine sweat glands and pores of palmoplantar skin. PPP is considered to be a localized pustular psoriasis. Interleukin ( IL )‐8, IL ‐36γ and IL ‐36Ra play important roles in the pathogenesis of pustular psoriasis, but their role in PPP is unclear. Aim To evaluate IL ‐8, IL ‐36γ and IL ‐36Ra expression in PPP , and their relationship with acrosyringia and pustule formation. Methods mRNA expression was quantified in skin samples from patients with PPP ( n = 7), patients with psoriasis vulgaris ( PSV ; n = 8) and healthy controls ( HC s) ( n = 6) by reverse‐transcription–real‐time PCR . Protein expression was characterized by immunohistochemistry ( PPP , n = 17; PSV , n = 14; HCs, n = 12). Sweat ducts, including acrosyringia, were stained for epithelial membrane antigen ( EMA ). Results IL ‐8 mRNA and protein were markedly increased in PPP lesions compared with PSV lesions or HC skin. IL ‐36γ mRNA and protein were significantly more abundant in PPP lesions than in HC skin. IL ‐36Ra mRNA was significantly overexpressed in PPP lesions compared with HC skin, but there was no difference in IL ‐36Ra protein between PPP , PSV and HC s. IL ‐8 was abundantly expressed by neutrophils in PPP pustules, while IL 36Ra was localized in the keratinocytes of PPP , PSV and HC skin. IL ‐36γ and EMA were colocalized in cells surrounding PPP pustules, and IL ‐36γ was also expressed in sweat duct cells in the dermis. Conclusion IL ‐8, IL ‐36γ and IL ‐36Ra are overexpressed in PPP lesions. IL ‐8, IL ‐36γ and acrosyringia, rather than IL ‐36Ra, are associated with pustule formation in PPP .