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Alternative activation of hedgehog pathway induced by ultraviolet B radiation: preliminary study
Author(s) -
Lesiak A.,
SobolewskaSztychny D.,
Bednarski I. A.,
Wódz K.,
Sobjanek M.,
Woźniacka A.,
Narbutt J.
Publication year - 2018
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.13394
Subject(s) - ptch1 , gli1 , sonic hedgehog , smoothened , vismodegib , hedgehog signaling pathway , hedgehog , cancer research , biology , medicine , microbiology and biotechnology , signal transduction
Summary Background There is still much ambiguity in studies of Sonic hedgehog (Shh) pathways and its dysregulation. Some studies concerning the role of the Shh pathway in basal cell carcinoma ( BCC ) have been conducted, but there is a lack of studies about Shh pathway dysregulation under the influence of ultraviolet ( UV )B radiation. Aim To evaluate skin expression of Shh, Ptch1, Ptch2, Smo and Gli1 proteins in BCC s with and without the influence of UVB radiation. Methods In total, 34 healthy controls ( HC s) and 42 patients with nodular BCC were recruited into the study. Patients were divided into five groups (A–E), depending on UVB dose received and BCC status. In all skin specimens, expression of Shh, Ptch1, Ptch2, Smo and Gli1 protein was evaluated. Results Comparing the BCC group with the HC group, there was significantly higher expression of Shh, Ptch1, Ptch2, Smo and Gli1 proteins. Expression of Ptch2, Smo and Gli1 was increased in response to UVB doses of 3 MED (minimal erythema dose), whereas expression of Ptch1 and Shh was unaffected. Conclusion The lack of change in expression of Shh and Ptch1 after exposure to UVB suggests that the Shh pathway may be activated through a noncanonical pathway under the influence of strong UVB doses.