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Association of oxidative stress and dynamic thiol‐disulphide homeostasis with atopic dermatitis severity and chronicity in children: a prospective study
Author(s) -
Uysal P.,
Avcil S.,
Neşelioğlu S.,
Biçer C.,
Çatal F.
Publication year - 2018
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.13219
Subject(s) - thiol , atopic dermatitis , oxidative stress , scorad , homeostasis , pathogenesis , medicine , stratum corneum , endocrinology , disease , chemistry , immunology , biochemistry , pathology , dermatology life quality index
Summary Background Oxidative stress ( OS ) has an important effect on the pathogenesis of atopic dermatitis ( AD ). Thiols are antioxidants that regulate intracellular redox metabolism and protect keratinocytes against OS damage in the stratum corneum. Aim To investigate dynamic thiol‐disulphide homeostasis ( dTDH ) as a novel OS parameter in children with AD , and its relationship with disease severity and chronicity. Methods Severity of AD was determined by using the instruments SCOR ing Atopic Dermatitis ( SCORAD ) and Eczema Area And Severity Index ( EASI ) upon enrolment in the study ( SCORAD 1 and EASI 1 ) and after 1 year ( SCORAD 2 and EASI 2 ). Native thiol, total thiol and disulphide levels were measured as novel OS parameters, and the ratios of disulphide/native thiol, disulphide/total thiol and native/total thiol were calculated as dTDH . Results In the AD group, the serum disulphide level and the ratios of disulphide/native thiol and disulphide/total thiol were significantly lower than in healthy controls ( P = 0.01, P < 0.01 and P < 0.01, respectively). There was no significant association between OS parameters and disease severity ( P > 0.05). SCORAD 2 and EASI 2 were positively correlated with disulphide/native thiol ratio ( r = 0.29, P < 0.03 and r = 0.35, P < 0.01, respectively), whereas they were negatively correlated with the native/total thiol ratio ( r = −0.30, P = 0.02 for both). Conclusions Both OS and impaired dTDH were found to be related to childhood AD . None of the OS parameters was associated with AD severity. dTDH is a possible diagnostic tool to predict AD chronicity.