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Comprehensive profiling of H‐Ras signalling in angiosarcoma endothelium
Author(s) -
Costa A.,
Bonner M.,
Arbiser J. L.
Publication year - 2017
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.13149
Subject(s) - angiosarcoma , profiling (computer programming) , signalling , medicine , endothelium , cancer research , pathology , biology , microbiology and biotechnology , computer science , operating system
Summary The MS 1/ SVR system, in which MS 1 represents immortalized endothelial cells and SVR represents MS 1 cells transformed with oncogenic human–rat sarcoma protein (H‐Ras), has been used for around 20 years as a valuable tool to study angiogenesis and carcinogenesis. Despite the use of these cells in numerous studies, a comprehensive profile of the signalling differences due to oncogenic H‐Ras transformation has not been performed previously. In this study, we profiled the well‐known MS 1 and SVR cell lines using a combination of both Western blot and gene chip assays.