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Potent effects of peracetylated (‐)‐epigallocatechin‐3‐gallate against hydrogen peroxide‐induced damage in human epidermal melanocytes via attenuation of oxidative stress and apoptosis
Author(s) -
Ning W.,
Wang S.,
Liu D.,
Fu L.,
Jin R.,
Xu A.
Publication year - 2016
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.12855
Subject(s) - oxidative stress , reactive oxygen species , chemistry , resveratrol , apoptosis , protein kinase a , p38 mitogen activated protein kinases , microbiology and biotechnology , gallate , melanocyte , hydrogen peroxide , antioxidant , biochemistry , kinase , cancer research , biology , melanoma , nuclear chemistry
Summary Background Damage to melanocytes induced by oxidative stress plays an important role in the pathogenesis of vitiligo. A polyphenol found in green tea, (‐)‐epigallocatechin‐3‐gallate ( EGCG ), exhibits certain antioxidative effects in the treatment of various diseases. The major problem that limits the clinical application of this polyphenol is its low bioavailability and stability. Peracetylated EGCG (Ac EGCG ), a fully acetylated derivative of EGCG , is more stable and bioavailable than EGCG , but the effects of its action on human epidermal melanocytes have not been elucidated. Aim To compare the protective effects of Ac EGCG and EGCG on hydrogen peroxide (H 2 O 2 )‐induced damage to human melanocytes. Methods Effects of AcEGCG and EGCG on human melanocytes were examined by measuring cell viability, levels of reactive oxygen species (ROS), the mitochondrial membrane potential (ΔΨm)and protein levels of caspase‐9, caspase‐3 and p38 mitogen‐activated protein kinase. Results Both Ac EGCG and EGCG decreased ROS generation, restored lost mitochondrial membrane potential and reduced H 2 O 2 ‐induced apoptosis in melanocytes. All of these effects were more pronounced with Ac EGCG than with EGCG . Furthermore, Ac EGCG effectively suppressed H 2 O 2 ‐induced p38 mitogen‐activated protein kinase phosphorylation, which has been suggested to contribute to melanocyte damage. Conclusions Ac EGCG is a more potent agent than EGCG for protection of melanocytes from oxidative damage.