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Nuclear magnetic resonance titration of the interaction between pemphigus vulgaris autoantibodies and REWVKFAKPCRE , a therapeutic desmoglein 3 peptide
Author(s) -
Lucchese A.,
Sinha A. A.
Publication year - 2016
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.12813
Subject(s) - desmoglein 3 , pemphigus vulgaris , autoantibody , desmoglein 1 , pemphigus , epitope , immunology , antibody , peptide , medicine , desmoglein , chemistry , biochemistry
Summary Background Pemphigus vulgaris ( PV ) is an autoimmune skin disease, the primary autoantigen of which is the desmosomal cadherin desmoglein (Dsg)3. The exact defin‐ition of Dsg3 epitopes and their relationship(s) to the pathophysiology of blister formation are important for the advancement of efforts to develop more effective and specific therapies for PV . Aim To characterize the binding of autoantibodies from patients with PV to a Dsg3 peptide, REWVKFAKPCRE , which shows therapeutic effectiveness but does not induce pathogenic antibodies. Methods We carried out a titration experiment of the reaction between PV autoantibodies and the peptide Dsg349‐60 REWVKFAKPCRE using nuclear magnetic resonance ( NMR ) spectroscopy. Results The interaction between Dsg349‐60 REWVKFAKPCRE and PV autoantibodies at concentrations of 20, 40 and 60 mg was found to involve R49 and A55 residues. Conclusions Our data seem to suggest that the REWVKFAKPCRE peptide may mimic epitopic Dsg3 extracellular sequences related to pathogenic autoantibodies.