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mRNA and protein expression of the angiogenesis‐related genes EDIL 3, AMOT and ECM 1 in mesenchymal stem cells in psoriatic dermis
Author(s) -
Niu X.,
Chang W.,
Liu R.,
Hou R.,
Li J.,
Wang C.,
Li X.,
Zhang K.
Publication year - 2016
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.12783
Subject(s) - angiogenesis , psoriasis , mesenchymal stem cell , biology , cancer research , microbiology and biotechnology , messenger rna , stem cell , pathology , medicine , immunology , gene , genetics
Summary Background Dermal microvasculature expansion and angiogenesis are prominent in psoriasis. Our previous microarray study showed that the angiogenesis‐related genes EDIL 3 (epidermal growth factor‐like repeats and discoidin I‐like domains 3), AMOT (angiomotin) and ECM 1 (extracellular matrix protein 1), had high expression levels in dermal mesenchymal stem cells ( DMSC s) from psoriatic skin lesions. Aim To investigate the m RNA and protein expressions of EDIL 3 , AMOT and ECM 1 in DMSC s derived from psoriatic skin in order to better determine the molecular mechanisms of angiogenesis in the skin. Methods DMSC s from 12 patients with psoriasis and 14 healthy controls ( HC s) were cultured to passage 3, and identified by morphology, immunophenotype and multipotential differentiation. The m RNA and protein expressions of EDIL 3 , AMOT , and ECM 1 in the DMSC s were determined using real‐time reverse transcription PCR and western blotting. Results DMSC s displayed spindle‐like morphology and surface protein expression, and were able to differentiate into osteoblasts, chondrocytes and adipocytes. m RNA expression analysis showed that EDIL 3, AMOT and ECM 1 were expressed at 2.54‐fold, 2.11‐fold, and 1.90‐fold higher levels, respectively, in psoriatic DMSC s compared with HC DMSC s (all P < 0.05). Protein analysis showed significantly (all P < 0.01) higher concentrations of EDIL 3, AMOT and ECM 1in the psoriasis group than in the HC group. Conclusions Our data demonstrate for the first time that expression of EDIL 3, AMOT and ECM 1 is altered in DMSC s in psoriasis, suggesting that EDIL 3, AMOT and ECM 1 are involved in the excessive angiogenesis and vasodilation observed in psoriasis.