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Skin dryness in apparently healthy human skin is associated with decreased expression of bleomycin hydrolase in the stratum corneum
Author(s) -
Son E. D.,
Kim Y.,
Joo K. M.,
Kim H. J.,
Lee E.,
Nam G. W.,
Cho E. G.,
Noh M.,
Chung J. H.,
Byun S. Y.,
Lee T. R.
Publication year - 2015
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.12520
Subject(s) - corporation , library science , management , center (category theory) , medicine , political science , sociology , chemistry , law , computer science , economics , crystallography
Summary Background Maintenance of water balance in the stratum corneum ( SC ) is determined by the content of intercellular lipids and natural moisturizing factors ( NMF s) in corneocytes. Aim To investigate the association between the NMF s and (pro)filaggrin and the proteases responsible for the processing of (pro)filaggrin to NMF s in the SC of hydrated and dry skin areas of healthy human subjects. Methods The SC hydration state and the transepidermal water loss ( TEWL ) were measured using a Corneometer and a Tewameter, respectively. Proteases, (pro)filaggrin and NMF s were extracted from SC samples obtained by tape‐stripping of the tested skin. Expression levels of (pro)filaggrin were determined by dot blotting and western blotting, and total NMF s by ultra‐high performance liquid chromatography. Expression of the proteases caspase‐14, calpain‐1 and bleomycin hydrolase was measured by western blotting. Results The levels of (pro)filaggrin were not significantly different between hydrated and dry skin, whereas the level of total NMF s was significantly reduced in dry skin. A negative correlation between (pro)filaggrin and NMF s was found in dry skin (Pearson correlation coefficient r = − 0.57, * P < 0.05). Bleomycin hydrolase expression was significantly decreased in the SC of dry skin. Conclusions These results suggest that the low hydration state of dry skin may be due to the reduction in (pro)filaggrin degradation caused by decreased bleomycin hydrolase expression.