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Diagnostic accuracy of BP 180 NC 16a and BP 230‐C3 ELISA in serum and saliva of patients with bullous pemphigoid
Author(s) -
Esmaili N.,
Mortazavi H.,
KamyabHesari K.,
Aghazadeh N.,
Daneshpazhooh M.,
Khani S.,
ChamsDavatchi C.
Publication year - 2015
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.12510
Subject(s) - medicine , bullous pemphigoid , dermatology , center (category theory) , library science , family medicine , immunology , antibody , computer science , chemistry , crystallography
Summary Background Bullous pemphigoid ( BP ) is a subepidermal blistering disease, characterized by autoantibodies directed against BP 180 and BP 230. Collecting saliva is an easy and painless way of obtaining biological samples, and can be used for diagnosis of autoimmune diseases. Aim To compare the diagnostic accuracy of serum and salivary BP 180‐ NC 16a and BP 230‐C3 in the initial diagnosis of BP . Methods We assessed 50 patients newly diagnosed with BP and 50 healthy controls. The diagnosis of BP was confirmed based on clinical, histopathological and immunofluorescence findings. Serum and saliva samples were collected from both groups, and BP 180 and BP 230 titres were assessed using commercially available ELISA kits. Results Using serum, the sensitivity of the serum BP 180 and BP 230 ELISA assays was 88% and 48%, respectively, and the specificity of both was 96%. Using saliva with the cutoff value proposed by the manufacturer, sensitivity was 56.2% and 14.6%, and specificity was 98% and 100%, respectively. Using the best calculated cutoff for saliva, sensitivity increased to 87.5% and 77.1%, and specificity to 96% and 62%, respectively. There was a significant correlation between serum and saliva BP 180 levels and the severity of skin disease. Both serum and saliva BP 230 levels were significantly higher in patients with mucosal involvement. Conclusion Serum BP 180 NC 16a ELISA is a sensitive and specific test for the initial diagnosis of BP , whereas serum BP 230‐C3 ELISA is highly specific, but less sensitive. Saliva may be a noninvasive and convenient alternative for use in the BP 180 NC 16a ELISA to diagnose BP .