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The combination of tumour necrosis factor‐α −308 A and interleukin‐10 −1082 G gene polymorphisms and increased serum levels of related cytokines: susceptibility to vitiligo
Author(s) -
Aydıngöz I. E.,
KanmazÖzer M.,
Gedikbaşi A.,
Vural P.,
DoğruAbbasoğlu S.,
Uysal M.
Publication year - 2015
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.12446
Subject(s) - vitiligo , pathogenesis , medicine , single nucleotide polymorphism , genotype , immunology , tumor necrosis factor alpha , interleukin , allele , cytokine , biology , gene , genetics
Summary Background The aetiopathogenesis of vitiligo is still under investigation. Aim To assess the role of single nucleotide polymorphisms ( SNP s) of the genes for tumour necrosis factor ( TNF )‐α, interleukin ( IL )‐6 and IL ‐10, as well as the serum levels of these three cytokines in the pathogenesis of vitiligo. Methods The study enrolled 105 patients with vitiligo, and 211 age‐ and sex‐matched controls. TNF ‐α (−308), IL ‐6 (−174) and IL ‐10 (−1082) promoter polymorphisms were investigated by Light SNiP assay and analysed by χ 2 test. Subsequently, the serum cytokine levels were assessed by ELISA and evaluated by M ann– W hitney U ‐test and K ruskal– W allis test. Results The frequency of the GG genotype of the IL ‐10 −1082 polymorphism was significantly higher in the vitiligo group compared with the healthy control group ( P = 0.02). Further investigations using combinations of these variant alleles detected a significant risk for vitiligo for individuals carrying both the IL ‐10 −1082 G and TNF ‐α −308 A alleles ( OR = 12.57, 95% CI 1.44–110.0, P < 0.01). Serum IL ‐10 and TNF ‐α levels were higher in the vitiligo group ( P = 0.001). In addition, TNF ‐α levels in patients with active disease were significantly higher than in patients with stable disease ( P < 0.02). Conclusions The concomitant presence of IL ‐10 −1082 G and TNF ‐α −308 A alleles significantly raises the risk for vitiligo. Furthermore, in accordance with these findings, serum IL ‐10 and TNF ‐α were also increased in this study, confirming the role of these cytokines in the pathogenesis of vitiligo.