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Differential expression of interleukin‐2 by anti‐ CD 3‐stimulated peripheral blood mononuclear cells in patients with psoriatic arthritis and patients with cutaneous psoriasis
Author(s) -
Bosè F.,
Capsoni F.,
Molteni S.,
Raeli L.,
Diani M.,
Altomare A.,
Garavaglia M.,
Garutti C.,
Frigerio E.,
Banfi G.,
Altomare G.,
Reali E.
Publication year - 2014
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.12251
Subject(s) - medicine , psoriasis , auteur theory , dermatology , art , art history , movie theater
Summary The differences in systemic T ‐cell responses between patients with psoriatic arthritis ( P s A ) and patients with cutaneous psoriasis ( P s) are still largely unknown. To determine differential features that could be used to distinguish P s A from P s, we compared the cytokine secretion profile of circulating T cells in patients with P s A , patients with cutaneous P s and control subjects. We determined T h1, T h2 and T h17 cytokine secretion of anti‐ CD 3‐stimulated peripheral blood mononuclear cells ( PBMC s) using a cytokine bead array. Normality of data distribution was assessed by the S hapiro– W ilk test, and statistical significance was calculated by the M ann– W hitney test. Phenotypic characterization of circulating T cells was performed by fluorescence‐activated cell sorting analysis. We found that the major systemic differences distinguishing P s A from cutaneous P s were the increased secretion of interleukin ( IL )‐2 by α‐ CD 3‐stimulated PBMC s and a higher percentage of circulating CD 3+ T cells expressing the proliferation marker CD 71 in P s A . These results indicate IL ‐2 as a possible biomarker of P s A , and suggest a role of circulating T cells with high proliferative capacity in the pathogenesis of P s A .