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Expression of pigment epithelium‐derived factor in human cutaneous appendages
Author(s) -
Li C.M.,
Li W.,
Man X.Y.,
Liu Z.G.,
Zheng M.
Publication year - 2013
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.12066
Subject(s) - pedf , inner root sheath , outer root sheath , biology , hair follicle , immunohistochemistry , microbiology and biotechnology , angiogenesis , pathology , immunology , cancer research , medicine
Summary Background Pigment epithelium‐derived factor ( PEDF ), a 50‐ kD a glycoprotein and a member of the serine protease inhibitor gene family, is well known as a potent endogenous inhibitor of angiogenesis. However, the expression of PEDF in human cutaneous appendages has not yet been determined. Aim To investigate the expression of PEDF in human cutaneous appendages. Methods Immunohistochemical staining was used to detect the expression of PEDF in human cutaneous appendages. Reverse transcriptase PCR , western blotting and indirect immunofluorescence were used to determine the m RNA and protein expression of PEDF on cells of the outer root sheath ( ORS ). A wound‐healing assay was used to determine the effect of different concentrations of PEDF on the migration of ORS cells. Results PEDF was expressed in the hair follicle (including epidermal matrix, inner root sheath, ORS and fibrous root sheath), sebaceous glands and eccrine sweat glands. Both protein and RNA expression of PEDF was detected, and expression was localized to both cytoplasm and nucleus of ORS cells. Both interleukin ( IL )‐4 and IL ‐17 at 25 ng/mL upregulated the expression of PEDF of ORS cells, with IL ‐4 having the greater effect. PEDF 50 ng/mL decreased migration of ORS cells. Conclusions PEDF is expressed in human cutaneous appendages and may play a modulatory role in the physiology of ORS cells.