Premium
Nicotinamide downregulates gene expression of interleukin‐6, interleukin‐10, monocyte chemoattractant protein‐1, and tumour necrosis factor‐α gene expression in HaCaT keratinocytes after ultraviolet B irradiation
Author(s) -
Monfrecola G.,
Gaudiello F.,
Cirillo T.,
Fabbrocini G.,
Balato A.,
Lembo S.
Publication year - 2013
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/ced.12018
Subject(s) - hacat , tumor necrosis factor alpha , monocyte , medicine , immunology , biology , cell culture , genetics
Summary Ultraviolet ( UV ) radiation has profound effects on human skin, causing sunburn, inflammation, cellular‐tissue injury, cell death, and skin cancer. Most of these effects are mediated by a number of cytokines produced by keratinocytes. In this study we investigated whether nicotinamide ( NCT ), the amide form of vitamin B 3, might have a protective function in reducing the expression of interleukin ( IL )‐1β, IL ‐6, IL ‐8, IL ‐10, monocyte chemoattractant protein ( MCP )‐1 and tumour necrosis factor ( TNF )‐α in UV ‐irradiated keratinocytes. H a C a T cells were treated with UVB in the presence or absence of NCT , and cytokine m RNA levels were examined by quantitative real‐time PCR . NCT significantly downregulated IL ‐6, IL ‐10, MCP ‐1 and TNF ‐α m RNA expression, whereas it did not exert any significant effect on IL ‐1β or IL ‐8 expression. Because of its ability to decrease these cytokine mediators after UV exposure, NCT is a possible therapy to improve or prevent conditions induced or aggravated by UV light.