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High‐fat diet‐induced obesity worsens TH2 immune response and immunopathologic characteristics in murine model of eosinophilic oesophagitis
Author(s) -
Silva Flávia Márcia de Castro e,
Oliveira Erick Esteves de,
Ambrósio Marcilene Gomes Evangelista,
Ayupe Marina Caçador,
Souza Viviane Passos de,
Gameiro Jacy,
Reis Daniele Ribeiro de Lima,
Machado Marco Antonio,
Macedo Gilson Costa,
Mattes Joerg,
Ferreira Ana Paula
Publication year - 2020
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.13533
Subject(s) - medicine , eosinophilia , cd11c , eosinophilic esophagitis , immunology , immune system , foxp3 , allergic inflammation , eosinophil , eotaxin , dendritic cell , inflammation , biology , disease , asthma , chemokine , biochemistry , gene , phenotype
Background Eosinophilic oesophagitis (EoE) is an emergent chronic immune‐mediated disease of the oesophagus, which affects both children and adults. It is clinically characterized by dysphagia, food impaction and oesophageal eosinophilia. Epidemiological studies indicate that obesity can worsen allergic symptoms; however, its effect on EoE immunopathological response has not been evaluated yet. This study aimed to assess the effect of obesity on allergic inflammation and T helper‐2 profile in an EoE experimental model. Methods Obesity was induced by high‐fat feeding. After 7 weeks of diet, male BALB/c mice were subcutaneously sensitized and orally challenged with OVA. Results Obesity itself induced a significant mast cell and eosinophil accumulation in the oesophagus, trachea, gut and lung. After allergy induction, this number was higher, when compared to lean‐allergic mice. Moreover, obese‐allergic mice showed higher remodelling area, in the oesophagus, associated with higher IL‐5 and TSLP mRNA expression. In contrast, FoxP3 and IL‐10 were less expressed in comparison with lean‐allergic mice. In addition, the amount of CD11c + MHCII + PDL1 + dendritic cells was reduced, while the number of CD11c + MHCII + CD80 + DCs and CD3 + CD4 + GATA 3 + IL‐4 + cells was increased in obese‐allergic mice in the spleen and lymph nodes when compared to lean‐allergic mice. Conclusion Obesity aggravated the immune histopathological characteristics in the EoE experimental model, which was associated with the reduction in the regulatory profile, and the increased inflammatory cells influx, related to the T H 2 profile. Altogether, the data provide new knowledge about obesity as a risk factor, worsening EoE symptoms, and contribute for future treatment strategies for this specific profile.