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Impaired immunoregulatory network of the CD 4 T lymphocytes in refractory asthma
Author(s) -
Fernandes Jamille S.,
Araujo Maria Ilma,
Almeida Tarcísio V. V. S.,
Andrade Lorena S.,
Lopes Diego M.,
Mello Luane M.,
Carvalho Edgar M.,
Cruz Álvaro A.,
Cardoso Luciana S.
Publication year - 2019
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.13351
Subject(s) - asthma , atopy , foxp3 , immunology , medicine , population , flow cytometry , refractory (planetary science) , immunoglobulin e , peripheral blood mononuclear cell , immune system , biology , antibody , in vitro , biochemistry , environmental health , astrobiology
Summary Background The immunopathogenesis of severe asthma has been associated with an inefficient regulatory response. There are a few studies about the CD 4 T cells profile among individuals with severe asthma refractory to treatment. Objective To evaluate the CD 4 T lymphocyte profile from individuals with severe asthma according to their response to treatment, relating to their atopy status and age of asthma onset. Methods We evaluated nineteen individuals with severe asthma refractory to treatment ( SAR ), 21 with well‐controlled or partly controlled severe asthma ( CSA ) and 23 with mild‐to‐moderate asthma ( MMA ). Lymphocytes were obtained from PBMC , and the frequency of expression of different molecules in this population was assessed using the flow cytometry. Results We observed the frequency of CD 4 + IFN ‐γ + T cells was higher in atopic individuals with SAR than with CSA . In addition, among the atopic and early‐onset asthma ( EOA ), the frequency of CD 4 + CTLA ‐4 + T cells was lower in the SAR group than the CSA group. In relation to non‐atopic and late‐onset asthma ( LOA ) phenotypes, we noted the frequency of CD 4 + FoxP3 + T cells was lower in individuals with SAR than with CSA . We also observed among the LOA patients, the frequency of CD 4 + TGF ‐β + T cells was decreased in SAR group than the in CSA group. Conclusion and Clinical Relevance Our data suggest that refractoriness to treatment in asthma is associated with a lower expression of distinct regulatory molecules by CD 4 T cells between those who are atopic and have EOA and those who are non‐atopic and have LOA . Thus, these results may contribute to the identification of new regulatory strategies to treat asthma according to their phenotypes.