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Gly m 5/Gly m 8 fusion component as a potential novel candidate molecule for diagnosing soya bean allergy in Japanese children
Author(s) -
Maruyama Nobuyuki,
Sato Sakura,
Cabanos Cerrone,
Tanaka Akira,
Ito Komei,
Ebisawa Motohiro
Publication year - 2018
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.13231
Subject(s) - allergy , food allergy , allergen , medicine , immunoglobulin e , recombinant dna , antibody , area under the curve , protein subunit , wheat allergy , oral food challenge , immunology , chemistry , biochemistry , gene
Summary Background Soya bean is a major food allergen in children. Component‐resolved diagnostics has improved the accuracy of diagnosing immunoglobulin E (IgE)‐mediated food allergies. Objective We aimed to develop a novel component for the diagnosis of soya bean allergy using recombinant technology. Methods Japanese paediatric patients with suspected soya bean allergy (n = 91) were included, and symptomatic (n = 40) and asymptomatic (n = 51) cases were divided through oral food challenge testing. Specific IgE ( sIgE ) antibodies to each recombinant allergen component were analysed by enzyme‐linked immunosorbent assay, and the diagnostic performances of the components were assessed by area under the receiver operating characteristic curves ( AUC ). Results Among the recombinant components, sIgE antibody levels to Gly m 8 showed the highest AUC (0.706). A combination of Gly m 8 and α’ subunit of Gly m 5, improved the diagnostic performance of the single components. Moreover, the N‐terminal extension region of α’ subunit of Gly m 5, which has low cross‐reactivity among the vicilins, showed higher diagnostic performance ( AUC 0.695) than the full‐length α’ subunit of Gly m 5 ( AUC 0.613). Based on these findings, we developed a fusion protein of Gly m 8 plus the extension region of α’ subunit of Gly m 5; this fusion protein was very efficient for diagnosing soya bean allergy ( AUC 0.801). Conclusion A fusion protein of Gly m 8 and the extension region of α’ subunit of Gly m 5 could potentially diagnose soya bean allergy in paediatric patients. Fusion proteins may be useful for producing novel allergen components with improved diagnostic value.