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Fcε RI expression and IgE binding by dendritic cells and basophils in allergic rhinitis and upon allergen immunotherapy
Author(s) -
Berings M.,
Gevaert P.,
De Ruyck N.,
Derycke L.,
Holtappels G.,
Pilette C.,
Bachert C.,
Lambrecht B. N.,
Dullaers M.
Publication year - 2018
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.13157
Subject(s) - immunoglobulin e , immunology , house dust mite , allergen , allergy , desensitization (medicine) , medicine , receptor , antibody
Summary Background In humans, both basophils and dendritic cells ( DC s) express the high‐affinity IgE receptor (Fcε RI ). Objective To gain more insight into the relation between serum IgE levels and Fcε RI expression and IgE binding by DC s and basophils in house dust mite ( HDM ) allergy and during subcutaneous immunotherapy ( SCIT ). Methods We measured Fcε RI , IgE and HDM allergen on DC s (conventional type 2 DC s, cDC 2s; plasmacytoid dendritic cells, pDC s) and basophils by flow cytometry in 22 non‐allergic vs 52 allergic subjects and upon HDM SCIT in 28 allergic subjects. IgE levels were measured in serum. Results Serum IgE correlated differentially with Fcε RI expression and IgE binding depending on cell type and allergic status. In non‐allergic subjects, Fcε RI /IgE surface densities increased with serum IgE to a significantly stronger degree on basophils compared to cDC 2s. By contrast, in allergic subjects Fcε RI /IgE surface densities increased with serum IgE to a slightly stronger degree on cDC 2s compared to basophils. In addition, the data set suggests sequential loading of IgE onto Fcε RI expressed by these cells (basophils> cDC 2s> pDC s). Finally, HDM SCIT induced a temporary increase in serum IgE, which was paralleled by a peak in Fcε RI and IgE on DC s, but not on basophils. Conclusions & Clinical Relevance This study provides a comprehensive insight into the relation between serum IgE and Fcε RI /IgE on basophils and DC subsets. The novel finding that HDM SCIT induces a temporary increase in Fcε RI expression on DC s, but not on basophils, can be an incentive for future research on the potential tolerogenic role of IgE/Fcε RI signalling in DC s in the setting of allergen immunotherapy.