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The COL 5A3 and MMP 9 genes interact in eczema susceptibility
Author(s) -
MargaritteJeannin P.,
Babron M.C.,
Laprise C.,
Lavielle N.,
Sarnowski C.,
Brossard M.,
Moffatt M.,
GagnéOuellet V.,
Etcheto A.,
Lathrop M.,
Just J.,
Cookson W. O.,
Bouzigon E.,
Demenais F.,
Dizier M.H.
Publication year - 2018
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.13064
Subject(s) - gene , snp , heritability , genetics , biology , single nucleotide polymorphism , genotype
Summary Background Genetic studies of eczema have identified many genes, which explain only 14% of the heritability. Missing heritability may be partly due to ignored gene–gene (G‐G) interactions. Objective Our aim was to detect new interacting genes involved in eczema. Methods The search for G‐G interaction in eczema was conducted using a two‐step approach, which included as a first step, a biological selection of genes, which are involved either in the skin or epidermis development or in the collagen metabolism, and as a second step, an interaction analysis of the selected genes. Analyses were carried out at both SNP and gene levels in three asthma‐ascertained family samples: the discovery dataset of 388 EGEA (Epidemiological study on the Genetics and Environment of Asthma) families and the two replication datasets of 253 SLSJ (Saguenay–Lac‐Saint‐Jean) families and 207 MRCA (Medical Research Council) families. Results One pair of SNP s, rs2287807 in COL 5A3 and rs17576 in MMP 9 , that were detected in EGEA at P ≤ 10 −5 showed significant interaction by meta‐analysis of EGEA , SLSJ and MRCA samples ( P = 1.1 × 10 −8 under the significant threshold of 10 −7 ). Gene‐based analysis confirmed strong interaction between COL 5A3 and MMP 9 ( P = 4 × 10 −8 under the significant threshold of 4 × 10 −6 ) by meta‐analysis of the three datasets. When stratifying the data on asthma, this interaction remained in both groups of asthmatic and non‐asthmatic subjects. Conclusion This study identified significant interaction between two new genes, COL 5A3 and MMP 9 , which may be accounted for by a degradation of COL 5A3 by MMP 9 influencing eczema susceptibility. Further confirmation of this interaction as well as functional studies is needed to better understand the role of these genes in eczema.