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Immunologic regulatory effects of human umbilical cord blood‐derived mesenchymal stem cells in a murine ovalbumin asthma model
Author(s) -
Kang S.Y.,
Park D.E.,
Song W.J.,
Bae B.R.,
Lee J.W.,
Sohn K.H.,
Lee H.S.,
Kang H.R.,
Park H.W.,
Chang Y.S.,
Choi S.J.,
Oh W.I.,
Min K.U.,
Cho S.H.
Publication year - 2017
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.12920
Subject(s) - ovalbumin , immunology , medicine , umbilical cord , spleen , mesenchymal stem cell , bronchoalveolar lavage , immunoglobulin e , cytokine , eosinophil , lung , asthma , antibody , pathology , antigen
Summary Background Mesenchymal stem cells ( MSC s) have multiple immunomodulatory properties and hold therapeutic potential for inflammatory diseases. However, the therapeutic and immunologic effects of human umbilical cord blood‐derived MSC s (hu MSC s) remain largely unexamined for asthma. Objective This study was to investigate the immunomodulatory properties of hu MSC s in an ovalbumin ( OVA )‐induced murine asthma model. Methods Mice were injected intraperitoneally with OVA and an aluminium hydroxide adjuvant. hu MSC s were administered via the tail vein (5×10 5 cells/100 uL) to female BALB /c mice prior to the initial OVA challenge. The effects of hu MSC s were assessed by investigating airway hyperresponsiveness, histological changes, inflammatory cell numbers, serum allergen‐specific antibodies, cytokine production in spleen, lung tissue, and bronchoalveolar lavage ( BAL ) fluid as well as expansion of regulatory T cells. Results Administration of hu MSC s significantly reduced methacholine bronchial hyperresponsiveness and eosinophil counts in BAL cells. Similarly, there was a significant decrease in serum OVA ‐specific IgE and IgG1 levels along with Th2 cytokine production ( IL ‐4, IL ‐5, and IL ‐13) in the lung and spleen tissues, whereas increased percentage of regulatory T cells was observed after treatment with hu MSC s. Conclusions Our results suggest that hu MSC treatment reduces OVA ‐induced allergic inflammation, which could be mediated by regulatory T cells.