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Dust mite allergen, glutathione S‐transferase, induces T cell immunoglobulin mucin domain‐4 in dendritic cells to facilitate initiation of airway allergy
Author(s) -
Mo L.H.,
Yang L.T.,
Zeng L.,
Xu L.Z.,
Zhang H.P.,
Li L.J.,
Liu J.Q.,
Xiao X.J.,
Zheng P.Y.,
Liu Z.G.,
Yang P.C.
Publication year - 2017
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.12800
Subject(s) - immunology , allergy , dendritic cell , mucin , house dust mite , biology , immunoglobulin e , allergen , mite , antibody , immune system , biochemistry , botany
Summary Background Allergens from dust mites play a critical role in the pathogenesis of airway allergy. The mechanism by which dust mite allergens induce allergic diseases is not fully understood yet. Objective This study tests a hypothesis that the eighth subtypes of Dermatophagoides farina allergen (Derf8) play an important role in the induction of airway allergy. Methods The protein of Derf8 was synthesized via molecular cloning approach. Dendritic cells ( DC ) were stimulated with Derf8 in the culture, and then, the expression of T cell immunoglobulin mucin domain 4 ( TIM 4) in dendritic cells ( DC ) was analysed. The role of Derf8 in the induction of airway allergy was evaluated with a mouse model. Results Exposure to Derf8 markedly induced the TIM 4 expression in DC s by modulating the chromatin at the TIM 4 promoter locus. Derf8 played a critical role in the expansion of the T helper 2 response in the mouse airway via inducing DC s to produce TIM 4. Administration with Derf8‐depleted dust mite extracts ( DME ) inhibited the allergic inflammation and induced regulatory T cells in mice with airway allergy. Conclusion Derf8 plays an important role in the initiation of dust mite allergy. Vaccination with Derf8‐deficient DME is more efficient to inhibit the dust mite allergic inflammation than using wild DME .