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B cells establish, but do not maintain, long‐lived murine anti‐peanut IgE a
Author(s) -
Moutsoglou D. M.,
Dreskin S. C.
Publication year - 2016
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.12715
Subject(s) - immunoglobulin e , adoptive cell transfer , splenocyte , immunology , cd20 , antibody , b cell , peanut allergy , chemistry , microbiology and biotechnology , biology , t cell , immune system
Summary Background Peanut allergy (PNA) has been reported to be transferred to tolerant recipients through organ and bone marrow (BM) transplantation. The roles T and B cells play in establishing, and the roles B cell subsets play in maintaining lifelong anti‐peanut IgE levels are unknown. Objectives To determine the cellular requirements for the transfer of murine PNA and to determine the role CD 20 + cells play in maintaining long‐lived anti‐peanut IgE levels. Methods We developed a novel adoptive transfer model to investigate the cellular requirements for transferring murine PNA. We also treated peanut‐allergic (PA) mice with anti‐ CD 20 antibody and measured IgE levels throughout treatment. Results Purified B220 + cells from PA splenocytes and purified CD 4 + cells from naïve (NA) splenocytes are the minimal requirements for the adoptive transfer of PNA. Prolonged treatment of allergic mice with anti‐ CD 20 antibody results in significant depletion of B cell subsets but does not affect anti‐peanut IgE levels, symptoms, or numbers of IgE antibody secreting cells (ASCs) in the BM. Adoptive transfer of BM and spleen cells from allergic donors treated with anti‐ CD 20 antibody does not result in the transfer of PNA in NA recipients, demonstrating that anti‐ CD 20 antibody treatment depletes B cells capable of differentiating into peanut‐specific IgE ASCs. Conclusions and Clinical Relevance Peanut allergy can be established in a NA hosts with B220 + cells from PA donors and CD 4 + cells from peanut‐NA donors. However, long‐term depletion of B220 + cells with anti‐ CD 20 antibody does not affect anti‐peanut IgE levels. These results highlight a novel role for B cells in the development of PNA and provide evidence that long‐lived anti‐peanut IgE levels may be maintained by long‐lived ASCs.