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Serological, genomic and structural analyses of the major mite allergen Der p 23
Author(s) -
Mueller G. A.,
Randall T. A.,
Glesner J.,
Pedersen L. C.,
Perera L.,
Edwards L. L.,
DeRose E. F.,
Chapman M. D.,
London R. E.,
Pomés A.
Publication year - 2016
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.12680
Subject(s) - allergen , immunoglobulin e , mite , population , immunology , chitin , serology , chemistry , biology , microbiology and biotechnology , allergy , antibody , biochemistry , medicine , ecology , environmental health , chitosan
Summary Background Der p 23 was recently identified in a European population as a major allergen and potentially a chitin binding protein. Objective This study sought to assess the importance of Der p 23 among other Dermatophagoides allergens in a North American population and to determine the structure for functional characterization. Methods IgE binding to Der p 23, Der p 1, Der p 2, Der p 5, Der p 7 and Der p 8 was measured by ELISA . RNA ‐seq data from D. pteronyssinus were compared as estimates of allergen expression levels. The structure was analysed by X‐ray crystallography and NMR . Results Despite a high prevalence of Der p 23, (75% vs. 87% and 79% for Der p 1 and Der p 2, respectively), the anti‐Der p 23 IgE levels were relatively low. The patient response to the 6 allergens tested was variable ( n = 47), but on average anti‐Der p 1 and anti‐Der p 2 together accounted for 85% of the specific IgE. In terms of abundance, the RNA expression level of Der p 23 is the lowest of the major allergens, thirty fold less than Der p 1 and sevenfold less than Der p 2. The structure of Der p 23 is a small, globular protein stabilized by two disulphide bonds, which is structurally related to allergens such as Blo t 12 that contain carbohydrate binding domains that bind chitin. Functional assays failed to confirm chitin binding by Der p 23. Conclusions and Clinical Relevance Der p 23 accounts for a small percentage of the IgE response to mite allergens, which is dominated by Der p 1 and Der p 2. The prevalence and amount of specific IgE to Der p 23 and Der p 2 are disproportionately high compared to the expression of other Dermatophagoides allergens.