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Different Bla‐g T cell antigens dominate responses in asthma versus rhinitis subjects
Author(s) -
Dillon M. B. C.,
Schulten V.,
Oseroff C.,
Paul S.,
Dullanty L. M.,
Frazier A.,
Belles X.,
Piulachs M.D.,
Visness C.,
Bacharier L.,
Bloomberg G. R.,
Busse P.,
Sidney J.,
Peters B.,
Sette A.
Publication year - 2015
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.12643
Subject(s) - immunology , antigen , asthma , elispot , medicine , allergy , biology , cd8
Summary Background and objective The allergenicity of several German cockroach (Bla‐g) antigens at the level of IgE responses is well established. However, less is known about the specificity of CD 4 + T H responses, and whether differences exist in associated magnitude or cytokine profiles as a function of disease severity. Methods Proteomic and transcriptomic techniques were used to identify novel antigens recognized by allergen‐specific T cells. To characterize different T H functionalities of allergen‐specific T cells, ELISPOT assays with sets of overlapping peptides covering the sequences of known allergens and novel antigens were employed to measure release of IL ‐5, IFN γ, IL ‐10, IL ‐17 and IL ‐21. Results Using these techniques, we characterized T H responses in a cohort of adult Bla‐g‐sensitized subjects, either with ( n = 55) or without ( n = 17) asthma, and nonsensitized controls ( n = 20). T cell responses were detected for ten known Bla‐g allergens and an additional ten novel Bla‐g antigens, representing in total a 5‐fold increase in the number of antigens demonstrated to be targeted by allergen‐specific T cells. Responses of sensitized individuals regardless of asthma status were predominantly T H 2, but higher in patients with diagnosed asthma. In asthmatic subjects, Bla‐g 5, 9 and 11 were immunodominant, while, in contrast, nonasthmatic‐sensitized subjects responded mostly to Bla‐g 5 and 4 and the novel antigen NBGA 5. Conclusions Asthmatic and nonasthmatic cockroach‐sensitized individuals exhibit similar T H 2‐polarized responses. Compared with nonasthmatics, however, asthmatic individuals have responses of higher magnitude and different allergen specificity.