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Increased local IgE production induced by common aeroallergens and phenotypic alteration of mast cells in Chinese eosinophilic, but not non‐eosinophilic, chronic rhinosinusitis with nasal polyps
Author(s) -
Cao P.P.,
Zhang Y.N.,
Liao B.,
Ma J.,
Wang B.F.,
Wang H.,
Zeng M.,
Liu W.H.,
Schleimer R. P.,
Liu Z.
Publication year - 2014
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.12304
Subject(s) - immunoglobulin e , immunology , eosinophil , nasal polyps , tryptase , eosinophilic , atopy , interleukin 5 , allergy , mast cell , eosinophil cationic protein , chymase , allergen , eosinophilia , medicine , antibody , pathology , cytokine , interleukin , asthma
Summary Background Eosinophilic and non‐eosinophilic chronic rhinosinusitis with nasal polyps ( CRS w NP ) display distinct patterns of inflammation. However, the pathogenic mechanisms underlying the heterogeneity of CRS w NP need further investigation. Objective To investigate local immunoglobulin E (IgE) production and phenotype of mast cells in eosinophilic and non‐eosinophilic CRS w NP in Chinese. Methods Total and specific IgE levels were analysed by means of the Immuno CAP system. The molecular steps involved in class‐switch recombination to IgE were investigated using RT ‐ PCR assays. Mast cell phenotypes, IgE‐ and high‐affinity IgE receptor (Fcε RI )‐positive cells, and allergen binding to specific IgE in sinonasal mucosa were determined by means of immunohistochemistry. Results Compared with controls and non‐eosinophilic CRS w NP , local total IgE levels were increased, and local specific IgE to common aeroallergens was more frequently found, in Chinese eosinophilic CRS w NP independent of atopy and without significant association with Staphylococcus aureus enterotoxins. The ε germline gene transcript was also more frequently detected in eosinophilic CRS w NP . The number of IgE‐ and Fcε RI ‐positive cells was increased in eosinophilic CRS w NP . Most IgE‐ and Fcε RI ‐positive cells were mast cells. Dust mite antigens could bind to IgE on mast cells in situ . The number of mast cells positive for both tryptase and chymase and activated mast cells was increased in eosinophilic CRS w NP and the number of activated mast cells positively correlated with local IgE level, eotaxin‐1 level, and eosinophil count in CRS w NP . Conclusions and Clinical Relevance The local IgE induced by common aeroallergens may mediate mast cell activation and contribute to subsequent eosinophilic inflammation in Chinese CRS w NP . This study offers a rationale for considering intervention strategies designed to target ‘local allergy’ in eosinophilic CRS w NP .