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Eosinophil activation and novel mediators in the aspirin‐induced nasal response in AERD
Author(s) -
Choi G.S.,
Kim J.H.,
Shin Y.S.,
Ye Y.M.,
Kim S.H.,
Park H.S.
Publication year - 2013
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/cea.12096
Subject(s) - eosinophil cationic protein , eosinophil , immunology , medicine , eosinophil granule proteins , nasal provocation test , asthma , allergy , allergen
Summary Background Eosinophil activation is the key feature of upper and lower airway inflammation in aspirin‐exacerbated respiratory disease ( AERD ). Objective To investigate the mechanism of eosinophil activation and identify novel inflammatory mediators using proteomics. Methods Thirty‐two asthmatic subjects were enrolled: 18 AERD patients who showed positive responses to the lysine‐aspirin nasal provocation test (L‐ ASA NPT ) and 14 aspirin‐tolerant asthma ( ATA ) patients who showed negative responses to the L‐ ASA NPT (control group). Nasal lavage fluid ( NLF ) was collected before (baseline), at 10, 30 and 60 min (early response), and at 3 h (late response) after the L‐ ASA NPT . Eosinophil cationic protein ( ECP ) and cysteinyl leucotriene (Cys LT ) levels were measured using an Immuno CAP system and ELISA respectively. To identify proteins involved in AERD , comparative proteomics was applied using NLF s collected before and after L‐ ASA NPT s in AERD patients. The clinical relevance of identified novel proteins was evaluated by ELISA using NLF s from the AERD and ATA groups. Results Eosinophil cationic protein and Cys LT levels both increased significantly during the early response in AERD . ECP levels increased until the late response in AERD , while Cys LT levels were not significantly increased during the late response. Proteomic analysis showed up‐regulation of apolipoprotein A1 (ApoA1), α2‐macroglobulin (α2M) and ceruloplasmin ( CP ), with significant increases in NLF of AERD patients, which was significantly higher in AERD patients with chronic rhinosinusitis. Significant correlations were noted between ECP and Cys LT , ApoA1, α2M and CP levels during the early response in AERD patients. Conclusion Eosinophil activation occurred in early and late responses after L‐ ASA NPT in upper airway mucosa of AERD patients, where ApoA1, α2M and CP as well as Cys LT may be involved in eosinophilic inflammation.