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Novel peptides with HIV‐1 reverse transcriptase inhibitory activity derived from the fruits of Quercus infectoria
Author(s) -
Seetaha Supaphorn,
Hangbua Supa,
Rattanasrisomporn Jatuporn,
Choowongkomon Kiattawee
Publication year - 2021
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.13770
Subject(s) - reverse transcriptase , peptide , human immunodeficiency virus (hiv) , enzyme , biology , rna , virology , hydrolysis , chemistry , genome , biochemistry , gene
The HIV‐1 reverse transcriptase (HIV‐1 RT), which is responsible for transcription of viral RNA genomes into DNA genomes, has become an important target for the treatment of patients with HIV infection. Hydrolyzed peptides from plants are considered a new source of potential drugs. In order to develop new effective inhibitors, peptides extracted from 111 Asian medicinal plants were screened against the HIV‐1 RT. The crude hydrolyzed peptides from the fruit peel of Quercus infectoria were selected for purification and peptide sequence determination by HPLC and LC‐MS. Two peptides of interest were synthesized, and an IC 50 test was performed to determine their ability to inhibit the HIV‐1 RT. The IC 50 values of the peptides AIHIILI and LIAVSTNIIFIVV were determined to be 274 ± 5.10 n m and 236.4 ± 7.07 n m , respectively. This indicated that these peptides could be further developed as potential HIV‐1 RT inhibitors.