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Synthesis and biological activity of Ub2 derived peptides as potential host‐directed antitubercular therapy
Author(s) -
Singh Shalini,
Yabaji Shivraj M.,
Ali Rafat,
Srivastava Kishore K.,
Haq Wahajul
Publication year - 2019
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.13508
Subject(s) - phagolysosome , chemistry , circular dichroism , peptide , lysosome , biochemistry , phagosome , cytosol , cationic polymerization , proteolysis , mycobacterium , biophysics , intracellular , microbiology and biotechnology , bacteria , biology , enzyme , organic chemistry , genetics
Abstract The correlation of mycobactericidal property of macrophages with its potential to deliver bacteria to hydrolytic lysosomes, augmented with ubiquitin‐derived peptides (Ub2), activates the process of autophagy. This leads to the formation of phagolysosomes supported by factor involving increased cationic charges which regulate the acidic pH causing elimination of Mycobacterium . To better understand this interaction of cationic‐rich ubiquitin‐derived peptides with mycobacteria and to identify putative mycobacterial intrinsic resistance mechanisms for phagolysosome formation, we have synthesized a new series of Ub2 peptides, wherein the Gly residues are replaced with azaGly with the aim to improve metabolic stability. In addition to that a new methodology is reported for the synthesis of heteroaryl tethered peptides using azaGly as a linker. We have demonstrated that positive puncta (directly proportional to the acidification of lysosome) in cytosol was significantly increased after 6 hours on the treatment of macrophage with Ub2 peptide derivatives ( 1 , 6 , 10, and 11 ) causing the higher intensity of lysosome observed through LysoTracker Red Dye. The circular dichroism spectral studies are carried out in water and water: TFE mixture and demonstrated that the Ub2 peptides have helix‐forming tendency in the presence of TFE . The recognizable intracellular killing of Mycobacterium tuberculosis by Ub2 peptides provides a new approach for host‐directed therapy.