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Whole‐cell biocatalysis platform for gram‐scale oxidative dearomatization of phenols
Author(s) -
Baker Dockrey Summer A.,
Doyon Tyler J.,
Perkins Jonathan C.,
Narayan Alison R. H.
Publication year - 2019
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.13443
Subject(s) - biocatalysis , chemistry , scalability , combinatorial chemistry , phenols , biochemical engineering , organic chemistry , computer science , catalysis , reaction mechanism , engineering , database
Technologies enabling new enzyme discovery and efficient protein engineering have spurred intense interest in the development of biocatalytic reactions. In recent years, whole‐cell biocatalysis has received attention as a simple, efficient, and scalable biocatalytic reaction platform. Inspired by these developments, we have established a whole‐cell protocol for oxidative dearomatization of phenols using the flavin‐dependent monooxygenase, TropB. This approach provides a scalable biocatalytic platform for accessing gram‐scale quantities of chiral synthetic building blocks.