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Design, synthesis, molecular modelling, and in vitro evaluation of tricyclic coumarins against Trypanosoma cruzi
Author(s) -
Coelho Gleicekelly Silva,
Andrade Josimara Souza,
Xavier Viviane Flores,
Sales Junior Policarpo Ademar,
Rodrigues de Araujo Barbara Caroline,
Fonseca Kátia da Silva,
Caetano Melissa Soares,
Murta Silvane Maria Fonseca,
Vieira Paula Melo,
Carneiro Claudia Martins,
Taylor Jason Guy
Publication year - 2019
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.13420
Subject(s) - benznidazole , trypanosoma cruzi , chagas disease , tricyclic , trypanocidal agent , chemistry , pharmacology , nifurtimox , drug , biochemistry , stereochemistry , biology , trypanosoma brucei , virology , parasite hosting , world wide web , computer science , gene
Chagas disease is caused by infection with the parasite protozoan Trypanosoma cruzi and affects about 8 million people in 21 countries in Latin America. The main form of treatment of this disease is still based on the use of two drugs, benznidazole and nifurtimox, which both present low cure rates in the chronic phase and often have serious side‐effects. Herein, we describe the synthesis of tricyclic coumarins that were obtained via NHC organocatalysis and evaluation of their trypanocidal activity. Molecular docking studies against trypanosomal enzyme triosephosphate isomerase (TIM) were carried out, as well as a theoretical study of the physicochemical parameters. The tricyclic coumarins were tested in vitro against the intracellular forms of Trypanosoma cruzi . Among the 18 compounds tested, 10 were more active than the reference drug benznidazole. The trypanocidal activity of the lead compound was rationalized by molecular docking study which suggested the strong interaction with the enzyme TIM by T. cruzi and therefore indicating a possible mode of action. Furthermore, the selectivity index of eight tricyclic coumarins with high anti‐ T . cruzi activity was above 50 and thus showing that these lead compounds are viable candidates for further in vivo assays.