Premium
Preparation and evaluation of effect on Escherichia coli and Staphylococcus aureus of radiolabeled ampicillin‐loaded graphene oxide nanoflakes
Author(s) -
Yurt Fatma,
Ersöz Onur Alp,
Harputlu Ersan,
Ocakoglu Kasim
Publication year - 2018
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/cbdd.13171
Subject(s) - ampicillin , escherichia coli , graphene , staphylococcus aureus , nuclear chemistry , fourier transform infrared spectroscopy , chemistry , oxide , in vivo , antibiotics , microbiology and biotechnology , bacteria , materials science , biochemistry , nanotechnology , biology , organic chemistry , chemical engineering , genetics , engineering , gene
Ampicillin is a one of effective antibiotics against Gram‐positive and Gram‐negative bacteria. This study aimed to label ampicillin‐loaded graphene oxide nanoflake ( AMP ‐ GO ) with 99m Tc and evaluate of its in vitro binding to Staphylococcus aureus and Escherichia coli . Firstly, ampicillin was loaded into graphene oxide nanoflake prepared. AMP ‐ GO was characterized by Fourier transform infrared spectroscopy ( FTIR ) and scanning electron microscope ( SEM ) techniques, and the amount of loaded ampicillin onto GO was determined by UV ‐Vis absorption spectroscopy. AMP and AMP ‐ GO were labeled with 99m Tc using stannous chloride reducing agent. Labeling efficiency of 99m Tc‐ AMP ‐ GO was found to be 97.66 ± 2.06%. 99m Tc‐ AMP ‐ GO has higher binding efficiencies to both S. aureus and E. coli than 99m Tc‐ AMP . 99m Tc‐ AMP ‐ GO could be promising candidate as agent infection nuclear imaging. Furthermore, in vivo studies of 99m Tc‐ AMP ‐ GO with infected rats are planned to be performed.